Transcriptome analysis of adenomyosis eutopic endometrium reveals molecular mechanisms involved in adenomyosis-related implantation failure and pregnancy disorders.

BACKGROUND: Women with adenomyosis are characterized by having defective decidualization, impaired endometrial receptivity and/or embryo-maternal communication, and implantation failure. However, the molecular mechanisms underlying adenomyosis-related infertility remain unknown, mainly because of the restricted accessibility and the difficult preservation of endometrial tissue in vitro. We have recently shown that adenomyosis patient-derived endometrial organoids, maintain disease-specific features while differentiated into mid-secretory and gestational endometrial phase, overcoming these research barriers and providing a robust platform to study adenomyosis pathogenesis and the associated molecular dysregulation related to implantation and pregnancy disorders. For this reason, we aim to characterize the dysregulated mechanisms in the mid-secretory and gestational endometrium of patients with adenomyosis by RNA-sequencing. METHODS: Endometrial organoids were derived from endometrial biopsies collected in the proliferative phase of women with adenomyosis (ADENO) or healthy oocyte donors (CONTROL) (n = 15/group) and differentiated into mid-secretory (-SECorg) and gestational (-GESTorg) phases in vitro. Following RNA-sequencing, the significantly differentially expressed genes (DEGs) (FDR < 0.05) were identified and selected for subsequent functional enrichment analysis and QIAGEN Ingenuity Pathway Analysis (IPA). Statistical differences in gene expression were evaluated with the Student's t-test or Wilcoxon test. RESULTS: We identified 1,430 DEGs in ADENO-SECorg and 1,999 DEGs in ADENO-GESTorg. In ADENO-SECorg, upregulated genes included OLFM1, FXYD5, and RUNX2, which are involved in impaired endometrial receptivity and implantation failure, while downregulated genes included RRM2, SOSTDC1, and CHAC2 implicated in recurrent implantation failure. In ADENO-GESTorg, upregulated CXCL14 and CYP24A1 and downregulated PGR were related to pregnancy loss. IPA predicted a significant inhibition of ID1 signaling, histamine degradation, and activation of HMGB1 and Senescence pathways, which are related to implantation failure. Alternatively, IPA predicted an inhibition of D-myo-inositol biosynthesis and VEGF signaling, and upregulation of Rho pathway, which are related to pregnancy loss and preeclampsia. CONCLUSIONS: Identifying dysregulated molecular mechanisms in mid-secretory and gestational endometrium of adenomyosis women contributes to the understanding of adenomyosis-related implantation failure and/or pregnancy disorders revealing potential therapeutic targets. Following experimental validation of our transcriptomic and in silico findings, our differentiated adenomyosis patient-derived organoids have the potential to provide a reliable platform for drug discovery, development, and personalized drug screening for affected patients.

La necesidad de la Enfermería Escolar: una revisión integradora de la literatura / The need for School Nursing: an integrative literature review

Introducción: La salud escolar debe velar por el bienestar integral de la comunidad escolar, fortaleciendo conductas de autocuidado y hábitos saludables a través de la promoción y prevención e investigación en salud. El abordaje holístico que define a la Enfermería hace que la inclusión de la enfermera escolar sea fundamental en los diferentes centros educativos. Objetivo: Analizar los beneficios del rol de la enfermería escolar en base a sus competencias para identificar la necesidad que supone la figura de la enfermería en los colegios.Metodología: Revisión integrativa de la literatura, cualitativa siguiendo las indicaciones de la declaración PRISMA. La búsqueda de las publicaciones se llevó a cabo en las bases de datos Web of Science y MedLine, seleccionándose aquellos artículos publicados en los últimos 5 años en inglés y español. Resultados: se incluyeron un total de 10 artículos. Su análisis muestra las competencias de la enfermera escolar, la perspectiva que tienen los docentes y los padres de la figura de la enfermera escolar, así como la necesidad que aporta a comunidad escolar. Conclusiones: La presencia de la enfermera escolar en los centros educativos garantiza tranquilidad y beneficios para la comunidad, no solo mediante actividades de prevención o asistenciales, sino también mediante estrategias de educación para la salud. Nuestra investigación ha recogido cómo pese a que existe un argumento firme y conciso sobre la importancia de esta figura continúa la lucha por su implantación. (AU)

Introduction: School health must ensure the comprehensive well-being of the school community, strengthening self-care behaviours and healthy habits through promotion and prevention and through health-related research. The holistic approach that defines Nursing makes the inclusion of the school nurse fundamental in the different educational centres.Objective: To analyse the benefits of the role of school nursing based on their remit to identify the need for nurses in schools. Methodology: Integrative review of the literature and qualitative review based on the indications of the PRISMA statement. The search for publications was carried out in the Web of Science and MedLine databases, selecting those articles published in the last 5 years in English and Spanish. Results: A total of 10 articles were included. Their analysis shows the remit of the school nurse, the perspective that teachers and parents have of the school nurse role, as well as the need it represents to the school community.Conclusions: The presence of the school nurse in educational centres ensures tranquillity and benefits for the community, not only through prevention or assistance activities, but also through health education strategies. Our research has revealed how, despite the fact that there is a firm and concise argument regarding the importance of this role, the fight for its establishment continues. (AU)

Human blastocysts uptake extracellular vesicles secreted by endometrial cells containing miRNAs related to implantation.

STUDY QUESTION: Are the extracellular vesicles (EVs) secreted by the maternal endometrium uptaken by human embryos and is their miRNA cargo involved in implantation and embryo development? SUMMARY ANSWER: Data suggest that EVs secreted by human endometrial epithelial cells are internalized by human blastocysts, and transport miRNAs to modulate biological processes related to implantation events and early embryo development. WHAT IS KNOWN ALREADY: Successful implantation is dependent on coordination between maternal endometrium and embryo, and EVs role in the required cell-to-cell crosstalk has recently been established. In this regard, our group previously showed that protein cargo of EVs secreted by primary human endometrial epithelial cells (pHEECs) is implicated in biological processes related to endometrial receptivity, embryo implantation, and early embryo development. However, little is known about the regulation of these biological processes through EVs secreted by the endometrium at a transcriptomic level. STUDY DESIGN, SIZE, DURATION: A prospective descriptive study was performed. Endometrial biopsies were collected from healthy oocyte donors with confirmed fertility on the day of oocyte retrieval, 36 h after the LH surge. pHEECs were isolated from endometrial biopsies (n = 8 in each pool) and cultured in vitro. Subsequently, conditioned medium was collected and EVs were isolated and characterized. Uptake of EVs by human blastocysts and miRNA cargo of these EVs (n = 3 pools) was analyzed. PARTICIPANTS/MATERIALS, SETTING, METHODS: EVs were isolated from the conditioned culture media using ultracentrifugation, and characterization was performed using western blotting, nanoparticle tracking analysis, and transmission electron microscopy. EVs were fluorescently labeled with Bodipy-TR ceramide, and their uptake by human blastocysts was analyzed using confocal microscopy. Analysis of the miRNA cargo of EVs was performed using miRNA sequencing, target genes of the most expressed miRNA were annotated, and functional enrichment analysis was performed. MAIN RESULTS AND THE ROLE OF CHANCE: EVs measured 100-300 nm in diameter, a concentration of 1.78 × 1011 ± 4.12 × 1010 (SD) particles/ml and expressed intraluminal protein markers Heat shock protein 70 (HSP70) and Tumor Susceptibility Gene 101 (TSG101), in addition to CD9 and CD81 transmembrane proteins. Human blastocysts efficiently internalized fluorescent EVs within 1-2 h, and more pronounced internalization was observed in the hatched pole of the embryos. miRNA-seq analysis featured 149 annotated miRNAs, of which 37 were deemed most relevant. The latter had 6592 reported gene targets, that in turn, have functional implications in several processes related to embryo development, oxygen metabolism, cell cycle, cell differentiation, apoptosis, metabolism, cellular organization, and gene expression. Among the relevant miRNAs contained in these EVs, we highlight hsa-miR-92a-3p, hsa-let-7b-5p, hsa-miR-30a-5p, hsa-miR-24-3p, hsa-miR-21-5p, and hsa-let-7a-5p as master regulators of the biological processes. LIMITATIONS, REASONS FOR CAUTION: This is an in vitro study in which conditions of endometrial cell culture could not mimic the intrauterine environment. WIDER IMPLICATIONS OF THE FINDINGS: This study defines potential biomarkers of endometrial receptivity and embryo competence that could be useful diagnostic and therapeutic targets for implantation success, as well as open insight further investigations to elucidate the molecular mechanisms implicated in a successful implantation. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by the Spanish Ministry of Education through FPU awarded to M.S.-B. (FPU18/03735), the Health Institute Carlos III awarded to E.J.-B. (FI19/00110) and awarded to H.F. by the Miguel Servet Program 'Fondo Social Europeo «El FSE invierte en tu futuro¼' (CP20/00120), and Generalitat Valenciana through VALi+d Programme awarded to M.C.C.-G. (ACIF/2019/139). The authors have no conflicts of interest to disclose. TRIAL REGISTRATION NUMBER: N/A.

Extracellular vesicles secreted by adenomyosis endometrial organoids contain miRNAs involved in embryo implantation and pregnancy.

RESEARCH QUESTION: Do extracellular vesicles secreted by the endometrium of women with adenomyosis contain miRNAs involved in adenomyosis-related infertility? DESIGN: A descriptive study using organoids from eutopic endometrium of women with adenomyosis (n = 4) generated and differentiated to secretory and gestational phases, in which miRNA cargo from extracellular vesicles secreted by these differentiated organoids in each phase was analysed by next-generation sequencing. miRNAs in secretory-extracellular vesicles and gestational-extracellular vesicles were selected based on the counts per million. miRNAs target genes in each phase were obtained from miRNet and gene ontology was used for enrichment analysis. RESULTS: miRNA sequencing identified 80 miRNAs in secretory-phase extracellular vesicles, including hsa-miR-21-5p, hsa-miR-24-3p, hsa-miR-26a-5p, hsa-miR-92a-3p, hsa-miR-92b-3p, hsa-miR-200c-3p and hsa-miR-423a-5p, related to adenomyosis pathogenesis and implantation failure. Further, 60 miRNAs were identified in gestational-phase extracellular vesicles, including hsa-miR-21-5p, hsa-miR-26a-5p, hsa-miR-30a-5p, hsa-miR-30c-5p, hsa-miR-222-3p and hsa-miR-423a-5p were associated with preeclampsia and miscarriage. Among the target genes of these miRNAs, PTEN, MDM4, PLAGL2 and CELF1, whose downregulation (P = 0.0003, P < 0.0001, P = 0.0002 and P = 0.0003, respectively) contributes to adenomyosis pathogenesis, and impaired early embryo development, leading to implantation failure and miscarriage, are highlihghted. Further, functional enrichment analyses of the target genes revealed their involvement in cell differentiation, proliferation, apoptosis, cell cycle regulation and response to extracellular stimuli. CONCLUSIONS: Eutopic endometrium in secretory and gestational phase from women with adenomyosis releases extracellular vesicles containing miRNAs involved in adenomyosis progression, impaired embryo implantation and pregnancy complications.

H3K4me3 mediates uterine leiomyoma pathogenesis via neuronal processes, synapsis components, proliferation, and Wnt/ß-catenin and TGF-ß pathways.

BACKGROUND: Uterine leiomyomas (UL) are the most common benign tumor in women of reproductive age. Their pathology remains unclear, which hampers the development of safe and effective treatments. Raising evidence suggests epigenetics as a main mechanism involved in tumor development. Histone modification is a key component in the epigenetic regulation of gene expression. Specifically, the histone mark H3K4me3, which promotes gene expression, is altered in many tumors. In this study, we aimed to identify if the histone modification H3K4me3 regulates the expression of genes involved in uterine leiomyoma pathogenesis. METHODS: Prospective study integrating RNA-seq (n = 48) and H3K4me3 CHIP-seq (n = 19) data of uterine leiomyomas versus their adjacent myometrium. Differentially expressed genes (FDR < 0.01, log2FC > 1 or < - 1) were selected following DESeq2, edgeR, and limma analysis. Their differential methylation and functional enrichment (FDR < 0.05) were respectively analyzed with limma and ShinyGO. RESULTS: CHIP-seq data showed a global suppression of H3K4me3 in uterine leiomyomas versus their adjacent myometrial tissue (p-value< 2.2e-16). Integrating CHIP-seq and RNA-seq data highlighted that transcription of 696/922 uterine leiomyoma-related differentially expressed genes (DEG) (FDR < 0.01, log2FC > 1 or < - 1) was epigenetically mediated by H3K4me3. Further, 50 genes were differentially trimethylated (FDR < 0.05), including 33 hypertrimethylated/upregulated, and 17 hypotrimethylated/downregulated genes. Functional enrichment analysis of the latter showed dysregulation of neuron-related processes and synapsis-related cellular components in uterine leiomyomas, and a literature review study of these DEG found additional implications with tumorigenesis (i.e. aberrant proliferation, invasion, and dysregulation of Wnt/ß-catenin, and TGF-ß pathways). Finally, SATB2, DCX, SHOX2, ST8SIA2, CAPN6, and NPTX2 proto-oncogenes were identified among the hypertrimethylated/upregulated DEG, while KRT19, ABCA8, and HOXB4 tumor suppressor genes were identified among hypotrimethylated/downregulated DEG. CONCLUSIONS: H3K4me3 instabilities alter the expression of oncogenes and tumor suppressor genes, inducing aberrant proliferation, and dysregulated Wnt/ß-catenin, and TGF-ß pathways, that ultimately promote uterine leiomyoma progression. The reversal of these histone modifications may be a promising new therapeutic alternative for uterine leiomyoma patients.

Exosomal Micro-RNAs as Intercellular Communicators in Idiopathic Pulmonary Fibrosis.

Communication between neighboring or distant cells is made through a complex network that includes extracellular vesicles (EVs). Exosomes, which are a subgroup of EVs, are released from most cell types and have been found in biological fluids such as urine, plasma, and airway secretions like bronchoalveolar lavage (BAL), nasal lavage, saliva, and sputum. Mainly, the cargo exosomes are enriched with mRNAs and microRNAs (miRNAs), which can be transferred to a recipient cell consequently modifying and redirecting its biological function. The effects of miRNAs derive from their role as gene expression regulators by repressing or degrading their target mRNAs. Nowadays, various types of research are focused on evaluating the potential of exosomal miRNAs as biomarkers for the prognosis and diagnosis of different pathologies. Nevertheless, there are few reports on their role in the pathophysiology of idiopathic pulmonary fibrosis (IPF), a chronic lung disease characterized by progressive lung scarring with no cure. In this review, we focus on the role and effect of exosomal miRNAs as intercellular communicators in the onset and progression of IPF, as well as discussing their potential utility as therapeutic agents for the treatment of this disease.

Deciphering the Role of Histone Modifications in Uterine Leiomyoma: Acetylation of H3K27 Regulates the Expression of Genes Involved in Proliferation, Cell Signaling, Cell Transport, Angiogenesis and Extracellular Matrix Formation.

Uterine leiomyoma (UL) is a benign tumor arising from myometrium (MM) with a high prevalence and unclear pathology. Histone modifications are altered in tumors, particularly via histone acetylation which is correlated with gene activation. To identify if the acetylation of H3K27 is involved in UL pathogenesis and if its reversion may be a therapeutic option, we performed a prospective study integrating RNA-seq (n = 48) and CHIP-seq for H3K27ac (n = 19) in UL vs MM tissue, together with qRT-PCR of SAHA-treated UL cells (n = 10). CHIP-seq showed lower levels of H3K27ac in UL versus MM (p-value < 2.2 × 10−16). From 922 DEGs found in UL vs. MM (FDR < 0.01), 482 presented H3K27ac. A differential acetylation (FDR < 0.05) was discovered in 82 of these genes (29 hyperacetylated/upregulated, 53 hypoacetylated/downregulated). Hyperacetylation/upregulation of oncogenes (NDP,HOXA13,COL24A1,IGFL3) and hypoacetylation/downregulation of tumor suppressor genes (CD40,GIMAP8,IL15,GPX3,DPT) altered the immune system, the metabolism, TGFß3 and the Wnt/ß-catenin pathway. Functional enrichment analysis revealed deregulation of proliferation, cell signaling, transport, angiogenesis and extracellular matrix. Inhibition of histone deacetylases by SAHA increased expression of hypoacetylated/downregulated genes in UL cells (p < 0.05). Conclusively, H3K27ac regulates genes involved in UL onset and maintenance. Histone deacetylation reversion upregulates the expression of tumor suppressor genes in UL cells, suggesting targeting histone modifications as a therapeutic approach for UL.

Role of MicroRNAs in Signaling Pathways Associated with the Pathogenesis of Idiopathic Pulmonary Fibrosis: A Focus on Epithelial-Mesenchymal Transition.

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive disease with high mortality and unclear etiology. Previous evidence supports that the origin of this disease is associated with epigenetic alterations, age, and environmental factors. IPF initiates with chronic epithelial lung injuries, followed by basal membrane destruction, which promotes the activation of myofibroblasts and excessive synthesis of extracellular matrix (ECM) proteins, as well as epithelial-mesenchymal transition (EMT). Due to miRNAs' role as regulators of apoptosis, proliferation, differentiation, and cell-cell interaction processes, some studies have involved miRNAs in the biogenesis and progression of IPF. In this context, the analysis and discussion of the probable association of miRNAs with the signaling pathways involved in the development of IPF would improve our knowledge of the associated molecular mechanisms, thereby facilitating its evaluation as a therapeutic target for this severe lung disease. In this work, the most recent publications evaluating the role of miRNAs as regulators or activators of signal pathways associated with the pathogenesis of IPF were analyzed. The search in Pubmed was made using the following terms: "miRNAs and idiopathic pulmonary fibrosis (IPF)"; "miRNAs and IPF and signaling pathways (SP)"; and "miRNAs and IPF and SP and IPF pathogenesis". Additionally, we focus mainly on those works where the signaling pathways involved with EMT, fibroblast differentiation, and synthesis of ECM components were assessed. Finally, the importance and significance of miRNAs as potential therapeutic or diagnostic tools for the treatment of IPF are discussed.

Integrative analysis of the DNA methylome and transcriptome in uterine leiomyoma shows altered regulation of genes involved in metabolism, proliferation, extracellular matrix, and vesicles.

Uterine leiomyomas (ULs) are the most common benign tumors in women of reproductive age. Despite the high prevalence, tumor pathology remains unclear, which hampers the development of safe and effective treatments. Epigenetic mechanisms appear to be involved in UL development, particularly via DNA methylation that regulates gene expression. We aimed to determine the relationship between DNA methylation and gene expression in UL compared with adjacent myometrium (MM) to identify molecular mechanisms involved in UL formation that are under epigenetic control. Our results showed a different DNA methylation profile between UL and MM, leading to hypermethylation of UL, and a different global transcriptome profile. Integration of DNA methylation and whole-transcriptome RNA-sequencing data identified 93 genes regulated by methylation, with 22 hypomethylated/upregulated and 71 hypermethylated/downregulated. Functional enrichment analysis showed dysregulated biological processes and molecular functions involved in metabolism and cell physiology, response to extracellular signals, invasion, and proliferation, as well as pathways related to uterine biology and cancer. Cellular components such as cell membranes, vesicles, extracellular matrix, and cell junctions were dysregulated in UL. In addition, we found hypomethylation/upregulation of oncogenes (PRL, ATP8B4, CEMIP, ZPMS2-AS1, RIMS2, TFAP2C) and hypermethylation/downregulation of tumor suppressor genes (EFEMP1, FBLN2, ARHGAP10, HTATIP2), which are related to proliferation, invasion, altered metabolism, deposition of extracellular matrix, and Wnt/ß-catenin pathway dysregulation. This confirms that key processes of UL development are under DNA methylation control. Finally, inhibition of DNA methyltransferases by 5-aza-2'-deoxycitidine increased the expression of hypermethylated/downregulated genes in UL cells in vitro. In conclusion, gene regulation by DNA methylation is implicated in UL pathogenesis, and reversion of this methylation could offer a therapeutic option for UL. © 2022 The Pathological Society of Great Britain and Ireland.

Curcumin Prevents the Glycation of Tricarboxylic Acid Cycle and Cell Respiration Proteins in the Heart of Mice Fed with a High-fructose Diet.

BACKGROUND: A high fructose diet (HFD) induces protein glycation. The latter is related to a higher risk of cardiovascular disease. Curcumin is a natural pleiotropic compound that may possess antiglycant properties. OBJECTIVE: The study aims to analyze the effect of curcumin on the content of glycated proteins in the hearts of 6-week-old mice fed with a HFD for 15 weeks. METHODS: Mice were allocated into four groups (n = 6/group): a control group that received a standard diet (CT); a group that received 30% w/v fructose in water (F); a group that received 0.75% w/w curcumin supplemented in food (C); a group that received 30% w/v fructose in water and 0.75% w/w curcumin supplemented in food (F+C). The content of glycated proteins in the heart was determined by Western Blot (whereas the spots were detected by 2D-PAGE) using anti-AGE and anti-CML antibodies. Densitometric analysis was performed using the ImageLab software. Glycated proteins were identified by MALDI-TOF-MS, and an ontological analysis was performed in terms of biological processes and molecular function based on the STRING and DAVID databases. RESULTS: Fourteen glycated protein spots were detected, two of them with anti-AGE and the other 12 with anti- CML. In total, eleven glycated proteins were identified, out of which three had decreased glycation levels due to curcumin exposure. The identified proteins participate in processes such as cellular respiration, oxidative phosphorylation, lipid metabolism, carbohydrate metabolism, the tricarboxylic acid cycle (TAC), and the organization of intermediate filaments. CONCLUSION: Curcumin decreased the fructose-induced glycation level of the ACO2, NDUFS7, and DLAT proteins.

Proteomic analysis of extracellular vesicles secreted by primary human epithelial endometrial cells reveals key proteins related to embryo implantation.

BACKGROUND: Successful implantation is dependent on coordination between maternal endometrium and embryo, and the role of EVs in the required cross-talk cell-to-cell has been recently established. In this regard, it has been reported that EVs secreted by the maternal endometrium can be internalized by human trophoblastic cells transferring their contents and enhancing their adhesive and invasive capacity. This is the first study to comprehensively evaluate three EV isolation methods on human endometrial epithelial cells in culture and to describe the proteomic content of EVs secreted by pHEECs from fertile women. METHODS: Ishikawa cells and pHEECs were in vitro cultured and hormonally treated; subsequently, conditioned medium was collected and EVs isolated. Ishikawa cells were used for the comparison of EVs isolation methods ultracentrifugation, ExoQuick-TC and Norgen Cell Culture Media Exosome Purification Kit (n = 3 replicates/isolation method). pHEECs were isolated from endometrial biopsies (n = 8/replicate; 3 replicates) collected from healthy oocyte donors with confirmed fertility, and protein content of EVs isolated by the most efficient methodology was analysed using liquid chromatography-tandem mass spectrometry. EV concentration and size were analyzed by nanoparticle tracking analysis, EV morphology visualized by transmission electron microscopy and protein marker expression was determined by Western blotting. RESULTS: Ultracentrifugation was the most efficient methodology for EV isolation from medium of endometrial epithelial cells. EVs secreted by pHEECs and isolated by ultracentrifugation were heterogeneous in size and expressed EV protein markers HSP70, TSG101, CD9, and CD81. Proteomic analysis identified 218 proteins contained in these EVs enriched in biological processes involved in embryo implantation, including cell adhesion, differentiation, communication, migration, extracellular matrix organization, vasculature development, and reproductive processes. From these proteins, 82 were selected based on their functional relevance in implantation success as possible implantation biomarkers. CONCLUSIONS: EV protein cargos are implicated in biological processes related to endometrial receptivity, embryo implantation, and early embryo development, supporting the concept of a communication system between the embryo and the maternal endometrium via EVs. Identified proteins may define new biomarkers of endometrial receptivity and implantation success.

Histone deacetylase inhibition by suberoylanilide hydroxamic acid: a therapeutic approach to treat human uterine leiomyoma.

OBJECTIVE: To evaluate the effect of inhibition of histone deacetylases (HDACs) by suberoylanilide hydroxamic acid (SAHA) treatment of human uterine leiomyoma primary (HULP) cells in vitro on cell proliferation, cell cycle, extracellular matrix (ECM) formation, and transforming growth factor ß3 (TGF-ß3) signaling. DESIGN: Prospective study comparing uterine leiomyoma (UL) vs. adjacent myometrium (MM) tissue and cells with or without SAHA treatment. SETTING: Hospital and university laboratories. PATIENT(S): Women with UL without any hormone treatment. INTERVENTION(S): Myomectomy or hysterectomy surgery in women for leiomyoma disease. MAIN OUTCOME MEASURE(S): HDAC activity was assessed by enzyme-linked immunosorbent assay, and gene expression was assessed by quantitative real-time polymerase chain reaction. Effects of SAHA on HULP cells were analyzed by CellTiter (Promega, Madison, Wisconsin), Western blot, and quantitative real-time polymerase chain reaction. RESULT(S): The expression of HDAC genes (HDAC1, fold change [FC] = 1.65; HDAC3, FC = 2.08; HDAC6, FC = 2.42) and activity (0.56 vs. 0.10 optical density [OD]/h/mg) was significantly increased in UL vs. MM tissue. SAHA decreased HDAC activity in HULP cells but not in MM cells. Cell viability significantly decreased in HULP cells (81.68% at 5 µM SAHA, 73.46% at 10 µM SAHA), but not in MM cells. Proliferating cell nuclear antigen expression was significantly inhibited in SAHA-treated HULP cells (5 µM SAHA, FC = 0.556; 10 µM SAHA, FC = 0.622). Cell cycle markers, including C-MYC (5 µM SAHA, FC = 0.828) and CCND1 (5 µM SAHA, FC = 0.583; 10 µM SAHA, FC = 0.482), were significantly down-regulated after SAHA treatment. SAHA significantly inhibited ECM protein expression, including FIBRONECTIN (5 µM SAHA, FC = 0.815; 10 µM SAHA, FC = 0.673) and COLLAGEN I (5 µM SAHA, FC = 0.599; 10 µM SAHA, FC = 0.635), in HULP cells. TGFß3 and MMP9 gene expression was also significantly down-regulated by 10 µM SAHA (TGFß3, FC = 0.596; MMP9, FC = 0.677). CONCLUSION(S): SAHA treatment inhibits cell proliferation, cell cycle, ECM formation, and TGF-ß3 signaling in HULP cells, suggesting that histone deacetylation may be useful for treatment of UL.

5-aza-2'-deoxycitidine inhibits cell proliferation, extracellular matrix formation and Wnt/ß-catenin pathway in human uterine leiomyomas.

BACKGROUND: Uterine leiomyoma is a benign tumor with unclear pathogenesis and inaccurate treatment. This tumor exhibits altered DNA methylation related to disease progression. DNMT inhibitors as 5-aza-2'-deoxycytidine (5-aza-CdR), have been suggested to treat tumors in which DNA methylation is altered. We aimed to evaluate whether DNA methylation reversion with 5-aza-CdR reduces cell proliferation and extracellular matrix (ECM) formation in uterine leiomyoma cells to provide a potential treatment option. METHODS: Prospective study using uterine leiomyoma and adjacent myometrium tissues and human uterine leiomyoma primary (HULP) cells (n = 16). In tissues, gene expression was analyzed by qRT-PCR and DNMT activity by ELISA. Effects of 5-aza-CdR treatment on HULP cells were assessed by CellTiter, western blot, and qRT-PCR. RESULTS: DNMT1 gene expression was higher in uterine leiomyoma vs myometrium. Similarly, DNMT activity was greater in uterine leiomyoma and HULP cells (6.5 vs 3.8 OD/h/mg; 211.3 vs 63.7 OD/h/mg, respectively). After 5-aza-CdR treatment on HULP cells, cell viability was reduced, significantly so at 10 µM (85.3%). Treatment with 10 µM 5-aza-CdR on HULP cells significantly decreased expression of proliferation marker PCNA (FC = 0.695) and of ECM proteins (COLLAGEN I FC = 0.654; PAI-1, FC = 0.654; FIBRONECTIN FC = 0.733). 5-aza-CdR treatment also decreased expression of Wnt/ß-catenin pathway final targets, including WISP1 protein expression (10 µM, FC = 0.699), c-MYC gene expression (2 µM, FC = 0.745 and 10 µM, FC = 0.728), and MMP7 gene expression (5 µM, FC = 0.520 and 10 µM, FC = 0.577). CONCLUSIONS: 5-aza-CdR treatment inhibits cell proliferation, ECM formation, and Wnt/ß-catenin signaling pathway targets in HULP cells, suggesting that DNA methylation inhibition is a viable therapeutic target in uterine leiomyoma.

Vitamin D as an effective treatment in human uterine leiomyomas independent of mediator complex subunit 12 mutation.

OBJECTIVE: To study whether vitamin D (VitD) inhibits cell proliferation and Wnt/ß-catenin and transforming growth factor-ß (TGFß) signaling pathways in uterine leiomyomas independent of mediator complex subunit 12 (MED12) mutation status. DESIGN: Prospective study comparing leiomyoma vs. myometrial tissues and human uterine leiomyoma primary (HULP) cells treated with or without VitD and analyzed by MED12 mutation status. SETTING: Hospital and university laboratories. PATIENT(S): Women with uterine leiomyoma without any treatment (n = 37). INTERVENTION(S): Uterine leiomyoma and myometrium samples were collected from women undergoing surgery because of symptomatic leiomyoma pathology. MAIN OUTCOME MEASURE(S): Analysis of Wnt/ß-catenin and TGFß pathways and proliferation by quantitative real-time polymerase chain reaction in leiomyoma and myometrial tissue as well as in VitD-treated HULP cells analyzed by Sanger sequencing. RESULTS: Sequencing data showed that 46% of leiomyomas presented MED12 mutation, whereas no mutations were detected in adjacent myometrium. Expression of Wnt/ß-catenin and TGFß pathway genes was significantly increased in MED12-mutated leiomyomas compared to matched myometrium; no significant differences were found in wild-type (WT) leiomyomas. In HULP cells, VitD significantly decreased PCNA expression of both MED12-mutated and WT groups. VitD treatment decreased WNT4 and ß-catenin expression in both groups compared to controls, with significance for WNT4 expression in MED12-mutated samples. Similarly, VitD significantly inhibited TGFß3 expression in cells from both groups. MMP9 expression also decreased. CONCLUSION: Despite molecular differences between MED12-mutated and WT leiomyomas, VitD inhibited Wnt/ß-catenin and TGFß pathways in HULP cells, suggesting VitD as an effective treatment to reduce proliferation and extracellular matrix formation in different molecular subtypes of uterine leiomyomas.

Educación continua en enfermería: Campus Virtual en Salud Pública en la Región de las Américas / Continuing Education in Nursing: Virtual Campus in Public Health in Americas Region / Educação continuada em enfermagem: Campus Virtual em Saúde Pública na Região das Américas

Introducción: educación continua es una inversión personal y profesional necesaria a lo largo de la vida profesional de los enfermeros, porque amplía y perfecciona conocimientos técnicos, cientícos y habilidades prácticas. Objetivo: describir la participación de enfermeros de la Región de las Américas en los cursos ofrecidos por el Campus Virtual de Salud Pública antes y al comienzo de la pandemia de la COVID-19, según género, edad, nivel educativo y lugar de trabajo. Método: estudio descriptivo cuantitativo. Se analizaron datos de los participantes de los cursos del periodo de enero del 2013 hasta junio del 2020 del Campus Virtual de Salud Pública. Población de estudio: enfermeros de la Región de las Américas que tomaron cursos virtuales. Se calcularon medidas de estadística descriptiva y proporciones de las variables sociodemográficas de los participantes de manera confidencial con autorización y credenciales para acceder datos en la plataforma. Resultados: a los 515 cursos virtuales ofrecidos por el Campus Virtual de Salud Pública se matricularon 368.018 enfermeros. Los porcentajes de enfermeros con certificado según el país de procedencia son los siguientes: Ecuador con 64,7%, México con 58,3%, Honduras con 52,9%, Paraguay con 48,6%, Colombia con 45,2%, Uruguay con 42,2%, Argentina con 38,0%, Chile con 22,7%, Perú con 21,3%, y Brasil con 9,7%. El 83,1% eran mujeres, el 75% tenía un nivel educativo universitario y el 40% tenía edades entre 26 y 35 años y el 47,1% trabajaba en hospitales. Conclusión: la pandemia de la COVID-19 refuerza la importancia de la educación continua virtual en la que los enfermeros pueden mejorar sus conocimientos, potencializar sus habilidades, y calicarse para los cambios en la práctica, la enseñanza y la investigación.

Introduction: continuing education is a necessary personal and professional investment throughout the professional life of nurses as it expands and perfects technical, scientific, and practical skills. Objective: to describe the participation of nurses from the Americas region in the courses offered by the Virtual Campus of Public Health before and at the beginning of the COVID-19 pandemic, according to gender, age, educational level, and workplace. Method: a quantitative descriptive study. Data from the participants of the courses from January 2013 to June 2020 of the Virtual Campus of Public Health were analyzed. Study population: nurses from the Americas Region who took virtual courses. Descriptive statistics measures and proportions of the sociodemographic variables of the participants were calculated confidentially with authorization and credentials to access data on the platform. Results: 368,018 nurses were enrolled in the 515 virtual courses offered by the Virtual Campus of Public Health. The percentages of certified nurses according to the country of origin are as follows: Ecuador with 64.7%, Mexico with 58.3%, Honduras with 52.9%, Paraguay with 48.6%, Colombia with 45.2%, Uruguay with 42.2%, Argentina with 38.0%, Chile with 22.7%, Peru with 21.3%, and Brazil with 9.7%. 83.1% were women, 75% had some degree of university education, 40% were between 26 and 35 years old, and 47.1% worked in hospitals. Conclusion: the COVID-19 pandemic reinforces the importance of virtual continuing education in which nurses can improve their knowledge, enhance their skills, and qualify for changes in practice, teaching, and research.

Introdução: a educação permanente é um investimento pessoal e profissional necessário ao longo da vida profissional do enfermeiro, pois amplia e aperfeiçoa as competências técnicas, científicas e práticas. Objetivo: descrever a participação de enfermeiros da Região das Américas nos cursos oferecidos pelo Campus Virtual de Saúde Pública antes e no início da pandemia COVID-19, segundo gênero, idade, escolaridade e local de trabalho. Método: estudo descritivo quantitativo. Foram analisados os dados dos participantes dos cursos de janeiro de 2013 a junho de 2020 do Campus Virtual de Saúde Pública. População do estudo: enfermeiros da Região das Américas que fizeram cursos virtuais. As medidas estatísticas descritivas e proporções das variáveis sociodemográficas dos participantes foram calculadas de forma condencial com autorização e credencial para acesso aos dados na plataforma. Resultados: dos 515 cursos virtuais oferecidos pelo Campus Virtual de Saúde Pública, foram cadastrados 368.018 enfermeiros. As percentagens de enfermeiros certificados segundo o país de origem são as seguintes: Equador com 64,7%, México com 58,3%, Honduras com 52,9%, Paraguai com 48,6%, Colômbia com 45,2%, Uruguai com 42,2%, Argentina com 38,0%, Chile com 22,7%, Peru com 21,3% e Brasil com 9,7%. 83,1% eram mulheres, 75% possuíam ensino superior completo, 40% tinham entre 26 e 35 anos e 47,1% trabalhavam em hospitais. Conclusão: a pandemia COVID-19 reforça a importância da educação permanente virtual em que os enfermeiros podem melhorar seus conhecimentos, aprimorar suas habilidades e se qualificar para mudanças na prática, no ensino e na pesquisa.

Educación interprofesional y seguridad del paciente: una revisión sistemática / Interprofessional Education and Patient Safety: A Systematic Review / Educação interprofissional e segurança do paciente: uma revisão sistemática

Introducción: la educación interprofesional (EIP) se define como una iniciativa educativa, formativa o docente que involucra a más de una profesión en el aprendizaje conjunto e interactivo. La formación del recurso humano en salud tiene como desafío plantear soluciones a la escasez de personal sanitario en el mundo y formar profesionales que tengan un desempeño óptimo. Objetivo: caracterizar las publicaciones relacionadas con la implementación de modelos en educación interprofesional y el impacto generado en algunos aspectos relacionados con la seguridad del paciente. Método: se realizó una revisión sistemática de la literatura científica en Pubmed, Science Direct, Scielo, LILACS y Google Scholar con siete estrategias de búsqueda garantizando la exhaustividad y reproducibilidad en las fases de la guía PRISMA. Resultados: se incluyeron n = 33 estudios de los cuales n = 19 (57,5%) fueron aplicados en el contexto hospitalario, n = 14 (42,4%) utilizaron metodologías cuasiexperimentales, de estos n = 4 estudios miden desenlaces directos relacionados con la seguridad del paciente, y en los otros n = 29 (87,8%) se encontró una mejora en la comunicación, percepción y conocimiento en los equipos de trabajo. Conclusión: el aprendizaje interprofesional mejora la comunicación y la interacción entre los profesionales de salud. No obstante, es difícil generalizar los resultados debido a la diversidad de metodologías de interacción interprofesional aplicadas en los estudios.

Introduction: interprofessional education (IPE) is an educational, training, or teaching initiative involving more than one profession in joint and interactive learning. The challenge of training human resources in health is to propose solutions to the shortage of health personnel globally and to train professionals who have optimal performance. Objective: characterize the publications related to the implementation of interprofessional education models and the impact generated in aspects related to patient safety. Method: a systematic review of the scientific literature was carried out in PubMed, Science Direct, SciElo, LILACS, and Google Scholar with seven search strategies guaranteeing completeness and reproducibility in the phases of the PRISMA guide. Results: n = 33 studies were included, of which n = 19 (57.5%) were applied in the hospital context, n = 14 (42.4%) used quasi-experimental methodologies, of these n = 4 studies measure direct outcomes related to patient safety, and in the other n = 29 (87.8%) an improvement was found in communication, perception, and knowledge in in the work teams. Conclusion: interprofessional learning improves communication and interaction between health professionals. However, it is difficult to generalize the results due to the diversity of interprofessional interaction methodologies applied in the studies.

Introdução: a educação interprofissional (EIP) é definida como uma iniciativa educacional, de treinamento ou de ensino que envolve mais de uma profissão na aprendizagem conjunta e interativa. O desafio da formação de recursos humanos em saúde é propor soluções para a carência de pessoal de saúde no mundo e formar profissionais com ótimo desempenho. Objetivo:caracterizar as publicações relacionadas à implantação de modelos na educação interprofissional e o impacto gerado em alguns aspectos relacionados à segurança do paciente. Método: foi realizada uma revisão sistemática da literatura científica no Pubmed, Science Direct, Scielo, LILACS e Google Scholar com sete estratégias de busca garantindo completude e reprodutibilidade nas fases do guia PRISMA. Resultados: n = 33 estudos foram incluídos, dos quais n = 19 (57,5%) foram aplicados no contexto hospitalar, n = 14 (42,4%) utilizaram metodologias quase-experimentais, destes n = 4 estudos medem resultados diretos relacionados com a segurança do paciente, e nos demais n = 29 (87,8%) houve melhora na comunicação, percepção e conhecimento nas equipes de trabalho. Conclusão: a aprendizagem interprofissional melhora a comunicação e a interação entre os profissionais de saúde. No entanto, é difícil generalizar os resultados devido à diversidade de metodologias de interação interprofssional aplicadas nos estudos

Antitumor Therapy under Hypoxic Microenvironment by the Combination of 2-Methoxyestradiol and Sodium Dichloroacetate on Human Non-Small-Cell Lung Cancer.

A hypoxic microenvironment is a hallmark in different types of tumors; this phenomenon participates in a metabolic alteration that confers resistance to treatments. Because of this, it was proposed that a combination of 2-methoxyestradiol (2-ME) and sodium dichloroacetate (DCA) could reduce this alteration, preventing proliferation through the reactivation of aerobic metabolism in lung adenocarcinoma cell line (A549). A549 cells were cultured in a hypoxic chamber at 1% O2 for 72 hours to determine the effect of this combination on growth, migration, and expression of hypoxia-inducible factors (HIFs) by immunofluorescence. The effect in the metabolism was evaluated by the determination of glucose/glutamine consumption and the lactate/glutamate production. The treatment of 2-ME (10 µM) in combination with DCA (40 mM) under hypoxic conditions showed an inhibitory effect on growth and migration. Notably, this reduction could be attributed to 2-ME, while DCA had a predominant effect on metabolic activity. Moreover, this combination decreases the signaling of HIF-3α and partially HIF-1α but not HIF-2α. The results of this study highlight the antitumor activity of the combination of 2-ME 10 µl/DCA 40 mM, even in hypoxic conditions.

Factores que influyen en el comportamiento de adherencia del paciente con Diabetes Mellitus Tipo 2 / Factors influencing adherence behavior of patients with Type 2 Diabetes Mellitus / Fatores influenciadores do comportamento de adesao do doente com Diabetes Mellitus Tipo 2 / Les facteurs influençant le comportement d'adhérence des patients atteints de diabète de type 2

Resumen: Objetivo: Analizar los factores que influyen en el comportamiento de Adherencia al tratamiento del paciente con Diabetes Mellitus tipo 2, en la Unidad de Especialidades de Enfermedades Crónicas en Villahermosa, en un periodo de 6 meses en el año 2018. Materiales y métodos: Se realizó un estudio de tipo cuantitativo, observacional, corte transversal y prospectivo, muestra de 106 pacientes con Diabetes Mellitus tipo 2 en la Unidad de Especialidades de Enfermedades Crónicas (UNEMEs Ec), en Villahermosa Tabasco. Resultados: Predomina el género femenino con 68 %; el rango de edad es de 51 a 55 años con 19.8 %. En cuanto al nivel de escolaridad 70.8% con primaria y 66 % se dedican a las labores del hogar. En cuanto a los factores que influyen en el comportamiento de adherencia, 88 % lo están con el paciente y 77% con el proveedor; 56 % coinciden en que el factor socioeconómico a veces influye y 49 % refieren que los relacionados con la terapia nunca influyen. En cuanto al comportamiento de adherencia, 47 % refieren tener ventaja para adherencia, 41 % en riesgo de no desarrollar comportamientos de adherencia y el nivel de conocimientos de los pacientes, fue intermedio en 49.1 %. Conclusiones: La población estudiada tiene riesgo de no desarrollar comportamientos de adherencia y cuentan con un nivel de conocimientos intermedio, lo cual implica la necesidad de valorar la intervención psicológica, así como el proceso de enseñanza del paciente, para impactar y/o reorientarlos hacia una mayor adherencia como elemento importante en su beneficio.

Summary: Objective: Analyze the factors that influence the behavior of Adherence to the treatment of the patient with Diabetes Mellitus type 2, in the Unit of Specialties of Chronic Diseases in Villahermosa, over a period of 6 months in 2018. Materials and methods: A quantitative, observational, cross-sectional and prospective study was carried out, with a sample of 106 patients with Diabetes Mellitus type 2 in the Unit of Specialties of Chronic Diseases (UNEMEs Ec), in Villahermosa Tabasco. Results: The female gender predominates with 68%; the age range is 51 to 55 years with 19.8%. Regarding the level of schooling, 70.8% have primary education and 66% are dedicated to housework. Regarding the factors that influence the adherence behavior, 88% are with the patient and 77% with the provider; 56% agree that the socioeconomic factor sometimes influences and 49% report that those related to therapy never influence. Regarding the adherence behavior, 47% reported having an advantage for adherence, 41% at risk of not developing adherence behaviors and the level of knowledge of the patients, was intermediate in 49.1%. Conclusions: The population studied has risk of not developing adherence behaviors and have an intermediate level of knowledge, which implies the need to assess the psychological intervention, as well as the patient's teaching process, to impact and / or reorient them towards greater adherence as an important element in their benefit.

Resumo: Objetivo: Analisar os fatores que influenciam o comportamento de adesão ao tratamento de doentes com Diabetes Mellitus Tipo 2, na Unidade de Cuidados de Especialidades Doenças Crónicas em Villahermosa, durante um período de 6 meses em 2018. Materiais e Métodos: Realizou-se um estudo quantitativo, de observado, de coorte transversal e prospetivo, amostra de 106 doentes com Diabetes Mellitus tipo 2 na Unidade de Cuidados de Especialidades de doenças crónicas (UNEMES Ec), em Villahermosa. Resultados: 68% da amostra é do sexo feminino, as idades são compreendidas entre 51 e 55 anos (19,8%). 70,8% da amostra apresenta como grau de escolaridade o ensino básico e 66% são trabalhadoras domésticas. Em relação aos fatores que influenciam o comportamento de adesão, 88% estão relacionados com o doente e 77% relacionados com o provedor de cuidados, 56% concordam que os fatores socioeconómicos influenciam por vezes essa adesão e 49% referem que os relacionados com a terapêutica não influenciam. Em relação à adesão, 47% relataram as vantagens da adesão e 41% os riscos de não desenvolver comportamentos de adesão. O nível de conhecimento dos doentes foi considerado intermediário com 49,1%. Conclusões: La population étudiée est á risque de ne pas développer de comportement d'observance et posséde un niveau de connaissance intermédiaire, ce qui implique la nécessité d'évaluer l'intervention psychologique, ainsi que le processus d'enseignement du patient, de les influencer et / ou de les réorienter vers une plus grande adhésion en tant qu'élément important de leur bénéfice.

Résumé: Objectif: Analyser les facteurs qui influencent sur le comportement de l'adhérence au traitement du patient diabétique de type 2, dans l'unité des spécialités des maladies chroniques á Villahermosa, sur une période de 6 mois en 2018. Matériels et méthodes: Une étude quantitative, observationnelle, transversale et prospective a été réalisée. Avec un échantillon de 106 patients atteints de diabéte sucré de type 2 de l'Unité des spécialités de maladies chroniques (UNEMEs Ec) á Villahermosa Tabasco. Résultats: Le sexe féminin predomine avec 68%; la tranche d'áge est de 51 á 55 ans avec 19,8%. En ce qui concerne le niveau de scolarité, 70,8% ont suivi un enseignement primaire et 66% sont consacrés aux travaux ménagers. En ce qui concerne les facteurs qui influencent le comportement d'adhésion, 88% sont avec le patient et 77% avec le prestataire; 56% sont d'accord pour dire que le facteur socio-économique influence parfois et 49% affirment que ceux liés á la thérapie n'influencent jamais. En ce qui concerne le comportement d'adhérence, 47% ont déclaré avoir un avantage pour l'observance, 41% á risque de ne pas développer de comportements d'adhésion et le niveau de connaissance des patients était intermédiaire chez 49,1%. Conclusions: plus de la moitié de la population étudiée est á risque de ne pas développer de comportement d'observance et posséde un niveau de connaissance intermédiaire, ce qui implique la nécessité d'évaluer l'intervention psychologique, ainsi que le processus d'enseignement du patient, de les influencer et / ou de les réorienter vers une plus grande adhésion en tant qu'élément important de leur bénéfice.

Negative interaction of Staphylococcus aureus on Fusarium falciforme growth ocular isolates in an in vitro mixed biofilm.

The interactions between prokaryotes and eukaryotes are abundant in nature. These microorganisms also interact in the human body. Fungal-bacteria interactions are present in many diseases. In this study, we evaluated the microbial interaction of Fusarium falciforme and Staphylococcus aureus developing mixed biofilm in vitro. When both microorganisms grew up together the mixed biofilm biomass decreased than F. falciforme monobiofilm biomass. S. aureus was able to interact and form aggregates over the mycelium and conidia surface of F. falciforme. Our results suggest that S. aureus could bind to colloidal chitin. On another hand, the supernatants from S. aureus biofilm and S. aureus-F. falciforme presented an antifungal effect over F. falciforme biofilm formation. Finally we found that the pH had an inhibitory effect over fungal biofilm formation. We concluded that S. aureus can affect the F. falciforme growth negatively in mixed biofilm involving factors like pH, supernatants compounds, anchor to chitin, and bacterial viability.

Estudio sobre la pérdida de cabello y las prácticas de cuidado entre los estudiantes de la Universidad de Manizales, Manizales (Colombia), 2016 / Study on hair loss and care practices among students of the Universidad de Manizales, Manizales (Colombia), 2016

Objetivo: el ambiente universitario sumado a las exigencias de tipo mental, físico y demás, representaría un factor de riesgo para el desarrollo de patologías capilares, que impliquen la pérdida de cabello, dentro de la población de estudiantes universitarios. El objetivo de la presente investigación es identificar la frecuencia del fenómeno de la caída de cabello en estudiantes regulares de la Universidad de Manizales, Manizales (Colombia), 2016. Materiales y métodos: estudio poblacional de corte transversal analítico, en una muestra de 330 estudiantes de la Universidad de Manizales. Se aplicó un instrumento que medía variables demográficas, variables de cuidado capilar y las escalas de alopecia androgénica Hamilton-Norwood y Ludwig. Resultados: edad promedio 21,8 años. Según las escalas empleadas el 55,7% de los estudiantes presentan caída de cabello. Se encontró enfermedad autoinmune en 7,6% de la muestra, siendo la más común el hipotiroidismo autoinmune en un 44%. El antecedente de alopecia se encontró en el 42,7% de los participantes. El nivel de estrés encontrado fue moderado en un 76,7% según cuestionario de estrés de examen de Cisco. Hubo relación significativa (p=0,000) entre el fenómeno de caída de cabello detectado y el programa académico cursado por el estudiante, siendo los más afectados contaduría 85%, derecho 75% y medicina 72%. Psicología reportó la menor cifra con 27,5%. El 84% de los estudiantes con enfermedad autoinmune presentan caída de cabello, el porcentaje de caída de cabello en quienes refirieron estrés de examen moderdo fue de 59,4%. Conclusiones: en la población del presente estudio se encuentra una proporción alta de caída de cabello, lo que hace urgente una intervención..(AU)

Objective: the university environment added to the demands of mental, physical and other types, would represent a risk factor for the development of hair pathologies, involving hair loss, within the population of university students. The objective of the present investigation is to identify the frequency of the phenomenon of hair loss in regular students of the University of Manizales, Manizales (Colombia), 2016. Materials and methods: analytical cross-sectional population study, in a sample of 330 students of the Universidad de Manizales. An instrument that measured demographic variables, capillary care variables and the androgenic alopecia scales Hamilton-Norwood and Ludwig was applied. Results: average age 21.8 years. According to the scales used, 55.7% of students show hair loss. Autoimmune disease was found in 7.6% of the sample, the most common being autoimmune hypothyroidism in 44%. The history of alopecia was found in 42.7% of the participants. The level of stress found was moderate by 76.7% according to the Cisco exam stress test. There was a significant relationship (p = 0.000) between the phenomenon of hair loss detected and the academic program taken by the student, with the most affected being accountancy with 85%, law with 75% law, and medicine 72%. Psychology reported the lowest with 27.5%. 84% of students with autoimmune disease have hair loss, the percentage of hair loss in those who reported moderate levels of stress was 59.4%. Conclusions: in the population of the present study there is a high proportion of hair loss, which makes an intervention urgent..(AU)